computer-assisted image analysis program nis-elements ar Search Results


3d sim image reconstruction  (Nikon)
99
Nikon 3d sim image reconstruction
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
3d Sim Image Reconstruction, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer assisted image analysis program niselements ar  (Nikon)
90
Nikon computer assisted image analysis program niselements ar
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Image Analysis Program Niselements Ar, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted semiautomated morphometry nis-br software  (Nikon)
90
Nikon computer-assisted semiautomated morphometry nis-br software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Semiautomated Morphometry Nis Br Software, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted morphometric system nis-elements br 3.0  (Nikon)
90
Nikon computer-assisted morphometric system nis-elements br 3.0
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Morphometric System Nis Elements Br 3.0, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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video-assisted computer program nis elements ar 4  (Nikon)
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Nikon video-assisted computer program nis elements ar 4
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Video Assisted Computer Program Nis Elements Ar 4, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer assisted sperm analysis (casa) nis-elements  (Nikon)
90
Nikon computer assisted sperm analysis (casa) nis-elements
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Sperm Analysis (Casa) Nis Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted area calculation nis-element 2.3  (Nikon)
90
Nikon computer-assisted area calculation nis-element 2.3
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Area Calculation Nis Element 2.3, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis-elements br microscope imaging software  (Nikon)
90
Nikon nis-elements br microscope imaging software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Br Microscope Imaging Software, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted microscope nis-elements br  (Nikon)
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Nikon computer-assisted microscope nis-elements br
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Microscope Nis Elements Br, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted image analysis system nikon nis elements  (Nikon)
90
Nikon computer-assisted image analysis system nikon nis elements
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Image Analysis System Nikon Nis Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer-assisted image analysis nis-elements br 3.1  (Nikon)
90
Nikon computer-assisted image analysis nis-elements br 3.1
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Image Analysis Nis Elements Br 3.1, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/computer-assisted image analysis nis-elements br 3.1/product/Nikon
Average 90 stars, based on 1 article reviews
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computer-assisted image analysis software nis- elements  (Nikon)
90
Nikon computer-assisted image analysis software nis- elements
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Computer Assisted Image Analysis Software Nis Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="100%" height="100%">

Journal: mBio

Article Title: The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense

doi: 10.1128/mBio.02716-18

Figure Lengend Snippet: Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm.

Article Snippet: 3D-SIM image reconstruction was performed in NIS-Elements 5.01 (Nikon) using the “stack reconstruction” algorithm with the following parameter settings.

Techniques: Time-lapse Microscopy, Expressing, Fluorescence, Microscopy, Epifluorescence Microscopy, Staining